Uncovering Hidden Partners: Detecting Co-Microbial Urinary Tract Infections among the Elderly

Mazin Z. Yashkur; Marwa H. Al-Khafaji

doi:10.32007/jfacmedbaghdad2416

المؤلفون

Mazin Z. Yashkur Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0005-9000-9694
Marwa H. Al-Khafaji Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0002-2042-6231

DOI:

https://doi.org/10.32007/jfacmedbaghdad2416

الكلمات المفتاحية:

الميكروبات المشتركة, اصابات المسالك البولية, المرضى كبار السن

الملخص

الخلفية: تحتل العدوى الميكروبية المشتركة جزءا كبيرا من التهابات المسالك البولية وخاصة عند كبار السن. ومثل هذه الإصابات تكون مخفية، وذلك لغلبة نوع واحد على النوع الآخر.

الأهداف: هدفت هذه الدراسة إلى الكشف عن مسببات العدوى المشتركة باستخدام تقنيات الزرع والجزيئي.

المرضى وطرق العمل: تم جمع مائة عينة ادرار سريرية من مرضى كبار السن يعانون من التهابات المسالك البولية، وتراوحت أعمار المرضى بين (55-75) سنة ونسبة الذكور والإناث هي (1:1). تم زرع هذه العينات على اوساط زرعية انتقائية وتفريقية. تم تنقية وتشخيص البكتريا المعزولة باستخدام نظام فايتيك. تم إجراء اختبار الحساسية للمضادات الحيوية لتقييم نمط المقاومة لمضادات الحياة. تم استخلاص الحمض النووي البكتيري وتم تصميم بادئات محددة لهذه الدراسة لتضخيم جينات المقاومة armA وrmtG بتقنية PCR.

النتائج: كانت الكليبسيلا الرئوية والإشريكية القولونية هي الميكروبات المشتركة السائدة التي تسبب التهاب المسالك البولية في المرضى المسنين. وكانت هذه الميكروبات المشتركة مقاومة للمضادات الحيوية aminoglycoside. يحمل كلا من K. pneumoniae و E. coliجين armA بينما كان جين rmtG هو السائد في K.pneumoniae فقط.

الاستنتاج: هناك فروق ذات دلالة إحصائية بين الجنسين في معدلات الإصابة المشتركة، وخاصة بين الإناث الذين تتراوح أعمارهم بين 55-60 و55-69 سنة. حددت اختبارات الحساسية للمضادات الحيوية والتحليل الجيني جينات المقاومة armA وrmtG هي السائدة في عزلات (E. coliوK.pneumoniae) ولكن تم تحديد وجود rmtG في K.pneumoniaeفقط. تؤكد الدراسة على الحاجة إلى استراتيجيات علاج خاصة بجنس المريض والمراقبة المستمرة لمقاومة المضادات الحيوية في عدوى المسالك البولية.

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