Role of Resistin Mutations in Chemotherapy Resistance: A Computational Analysis in Iraqi Women with Breast Cancer
DOI:
https://doi.org/10.32007/jfacmedbaghdad3148Keywords:
Breast cancer, Resistin, haploreg, swissmodel, in silicoAbstract
Introduction: Breast cancer is one of the most common types of cancer diagnosed in women, and there are multiple genetic and environmental causes involved in progression of this type of cancer. Methods: The study aimed to analyses some Single Nucleotide Polymorphisms (SNPs) that are harmful to the structure and function of Resistin (RETN) protein which is the target for chemotherapy in breast cancer. We employed a variety of computational methodologies to find SNPs that are harmful to the structure and function of the RETN protein and could cause chemoresistance in breast cancer patients. Haploreg, Regulome DB, Poly- Phen-2, Protparam, and SWISS-MODEL were among the in-silico methods used to do the computational research. Results: The findings reveal that mutations of rs1862513, rs3219175, rs34788323 and rs3745367 are important alterations in native RETN protein that may contribute to its chemoresistance and, ultimately, breast cancer. The study also provided three-dimensional structures of the native and mutant model of RETN protein. Conclusion: According to our results of in silico analysis, these SNPs may have an effect on the binding site of some transcription factors (TFs) which may attribute to the nature of transcription factors that could be affected by others TFs and activators.
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Copyright (c) 2025 Khadija A. Sahan, Zahraa A. Sahan, Thualfiqar Gh. Turki
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